Course Overview

Course Overview

The contents of the School comprise a diverse and all-encompassing programme, a mixture of lectures and laboratory practical work.

Highlights of 2026

Mina K. Chung: Atrial fibrillation from genes to therapeutics

Michal Cifra: Electric field effects on proteins: direct and indirect pathways

Mauro Giacca: RNA therapies for cardiac repair and regeneration

Brendan Koop: Fundamental principles of designing safe, effective, and usable systems for cardiac ablation via pulsed electric fields

Samo Mahnič: On the origin of bubbles in PFA

Helmut Pürerfellner: How PFA will change cardiac arrhythmia treatment

Matej Reberšek: Blood is not water

Mike Sano: It Begins At The End – How Clinical Goals and Constraints Affect Electroporation Device Development

Graham Wright: MRI characterization of PFA lesions


Laboratory and practical work

More details on the Practical work page

  • L1 – Gene electrotransfer using locally enhanced electric field
  • L2 – Visualization of local ablation zone distribution between two needle electrodes
  • L3 – Comparison of flow cytometry and spectrofluorometric measurements in cell permeabilization experiments
  • L4 – Triggering action potential and electroporation in excitable cells exposed to electric pulses
  • L5 – E. coli inactivation by pulsed electric fields in a continuous mode
  • L6 – Influence of the orientation of the electric field on the formation of the DNA membrane complex
  • L7 – Measurements of the induced transmembrane voltage with fluorescent dye di-8-ANEPPS
  • L8 – Influence of the orientation of the electric field on gene electrotransfer efficiency and cell viability
  • L9 – Modelling, visualizing, and tracking pH front formation in tissue phantoms
  • L10 – Simultaneous measurement of sarcomere shortening and calcium transients in primary rat cardiomyocytes exposed to electrical pulses
  • L11 – Visualizing gas bubble formation during pulsed field delivery
  • L12 – Visualization of cytoskeletal filaments with super-resolution structured illumination microscopy (SIM)
  • L13 – Electroporation for treatment of cardiac arrhythmias
  • L14 – Monitoring cell membrane depolarization due to electroporation using fluorescent plasma membrane potential indicator
  • L15 – Measurements of the induced transmembrane voltage with fluorescent dye ElectroFluor630
  • C1 – Treatment planning for electrochemotherapy and irreversible electroporation: optimization of voltage and electrode position
  • C2 – Numerical modeling of thermal effects during irreversible electroporation treatments
  • C3 – Molecular dynamics simulations of membrane electroporation
  • C4 – Numerical modeling of pulsed field ablation in swine ventricular myocardium
  • H1 – Measurement of electroporation pulses with oscilloscope, and voltage and current probes
  • H2 – Development of pulsed power generators for electroporation

More details on the Practical work page